Culturing and enumerating bacteria from soil samples. Isolation and biochemical characterization of halophiles from. A total of 50 water, sediments and soil samples were collected from maharlu salt lake in southern region of farsiran and subjected for isolation of the bioactive. If using the soil sample, thoroughly mix by vigorous shaking. Isolation and characterization of moderately halophilic. Abstract the aim of the study was to isolate moderately halophilic bacteria that produce proteolytic enzymes with industrial biotechnological value. Isolation and characterization of denitrifying halophilic bacteria from. Soil sample were collected at specified depths upto1012 cm using a soil corer. The remainder of each sample was preserved at 4 oc for further analysis. Halophilic and halotolerant microorganisms from soils.
Isolation and characterization of a phosphatesolubilizing. Isolation, screening and characterization of phosphate. However, despite these salt requirements, it has also been shown that moderately halophilic. Studies of two pigment producing halophilic bacteria from.
Isolation, characterization of halotolerant bacteria. Isolation of moderately halophilic bacteria from tunisian. A gramnegative, moderately halophilic, short rodshaped, aerobic bacterium with peritrichous flagellae, strain dqd230t, was isolated from a soil sample contaminated with crude oil from the daqing oilfield in heilongjiang province, northeastern china. The study focused on the isolation and identification of the halotolerant bacteria from three soil samples, collected from. Moreover, it may increase the crop productivity of coastal saline lands in bangladesh by transferring the salt tolerant genes into the plants. Slightly halophilic or halotolerant organisms require 16% 0. Isolation and biochemical characterization of halophiles. Isolation and characterization of two halophilic bacillus b. Isolation and characterization of two halophilic bacillus.
Isolation of antibiotic producing microorganisms by. Nagalakshmi department of microbiology, kanchi shri krishna college of arts and science, kilambi, kanchipuram631551, tn, india corresponding author abstract introduction phosphorus p is a major growthlimiting. Enrichment and isolation of bacteria enrichment cultures and techniques to. Isolation and characterization of two novel halotolerant catechol 2, 3dioxygenases from a halophilic bacterial consortium.
Phosphatesolubilizing bacteria psb function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants. Culturable diversity of halophilic bacteria in foreshore soils. Salinibacter will grow on modified growth medium mgm, a medium formulated around a salt water solution that mimics the composition of concentrated seawater, and a rich nutrient source 5 g l. Isolation and characterization of indole acetic acid. Assessment of the bacterial biodiversity of halophilic. Soil bacteria are an important part of a healthy soil ecosystem, playing roles in decomposition, nitrogen fixing, and nutrient cycling. Moderately halophilic bacteria can be easily isolated from a range of saline environments 1 including salterns 2 and salt lakes 3. Soil type feature cfug of dry soil richness shannons index evenness 1 sandy bare 3. Pdf isolation and identification of protease producing. Microorganisms are found throughout the environment. Isolation and characterization of halophilic soil fungi. Isolation and characterization of phosphate solubilizing. Isolation and characterization of phosphate solubilizing microbes from agricultural soil t. Dilution plates and warcup soil plates, two widely used methods of isolating soil microorganisms, are described by johnson and curl 1972.
The plant roots and soil samples were transported to the laboratory, and portions were immediately plated for rhizobacteria isolation. Isolation of phosphate solubilizing bacteria was performed by suspending 1g rhizosphere soil in 100ml of distilled water. Lab report on isolation of microorganism from soil. In the second technique, little soil granules were taken with a spatula. Production and characterization of orange pigment produced. The main aim of this study was to deal with isolation, characterization and identification iaa producing bacteria from paddy rice cultivated soil samples collected in riceshrimp farming system in salt affected areas of the mekong delta of vietnam.
Isolation and characterization of halophilic bacteria from. Actinomycetes are a group of grampositive bacteria most commonly isolated from soil but also from other environmental niches such as in marine sediments. Isolation, purification, and preservation of medium, supplemented with 10halophilic bacteria for isolation of halophilic bacteria,1. In addition, one gr oup can attempt to isolate bacteria from the soil sample. In the preliminary screening twelve halophilic bacterial isolates exhibited protease production. In this lab we have focus on isolation of bacteria from environment. Isolation of bacteria was carried out by serial dilution up to 108 and by directly placing soil granules 26 on both culture media mentioned above.
Ii osmoregulation in halophilic bacteria hans jorg kunte encyclopedia of life support systems eolss is also considered. Halophilic bacterium an overview sciencedirect topics. David burns, mike dyallsmith, in methods in microbiology, 2006. Halophilic and halotolerant bacteria were isolated from soil samples of a japanese salt field, an environment where salt concentrations vary annually. Isolation, taxonomy, and antagonistic properties of. Fifteen soil samples collected from the rice crop in the riceshrimp farming system of 5 different provinces within the mekong delta, vietnam. A total of 2 moderately halophilic bacteria were isolated from hypersaline soils with a cl content between 2.
Types and properties of some bacteria isolated from hypersaline soils. Excluding haloalkaliphiles, most haloarchaea and halophilic bacteria e. Isolation, molecular characterization and extracellular enzymatic activity of culturable halophilic bacteria from hypersaline natural habitats. Isolation of orange pigmentproducing bacteria the soil samples collected were serially diluted and plated on. Variable levels of cultivable halophilic actinomycetes were observed, ranging from 102 to 103 cfu per g dry weight of soil, which represented only 0. Characterization of 21 potential isolates by morphological.
A moderately halophilic bacterium was isolated from the sediment of daqiao saltern on the eastern coast of china, which also. The protease producing halophilic bacteria were isolated from the banks of different regions of the indian ocean near chennai. Isolation and characterization of bacteria isolated from. Screening of halophiles from various saline habitats of india led to isolation of 108 halophilic bacteria producing industrially important hydrolases amylases, lipases and proteases. Isolation of efficient phosphate solubilizing bacteria.
An orange colour bacterial strain that was grown on nutrient agar on normal incubation after about. Isolation of phosphate solubilizing bacteria psb from rhizosphere soil soil samples were prepared by inoculating 1. Soil sample were collected from upper layer of the farmland where maximum population of microorganism was concentrated. Clearly, moderately halophilic bacteria can survive in nonsaline soil environments and all of the slightly and moderately halophilic bacteria isolated are endosporeformers suggesting that they survive in nonsaline habitats in. Isolation of halophilic and alkalophilic actinobacteria. To establish a correlation with the soil composition. The selected culture strains of bacteria were then subjected to gram staining, spore staining and biochemical characterization tests and their results are shown. The focus will be on the export of compatible solutes via mechanosensitive channels to cope with stress caused by dilution of the medium e. Pdf isolation of halophilic bacteria associated with saline and. The basis of all selective isolation techniques is the provision of conditions particularly favourable to the organism concerned. Halophilic bacteria have evolved in a way they can resist rigid situations which their counterparts fail. Soil sample was colleceted at about 15cms root depth of plant. Soil sample were collected from surrounding villages of akola district. Isolation of halophilic bacteria associated with saline.
A greenish color was observed in ka16spiv and a pink color in ka16sk2hs cultures. Isolation, molecular characterization and extracellular. Homogenization of soil was carried out by keeping it on shaker for 30 minutes at 120 rpm at 27oc temperature. Screening of halophiles from various saline habitats, led to the isolation of 210 moderately halophilic bacteria producing industrially important hydrolases.
To establish a correlation with the soil composition and the halophilic bacteria found on these soils. A new medium for the isolation and enrichment of halophilic. Isolating microorganisms by screening for antibiotic resistance in this study. Isolation of halophilic and anaerobic bacteria by progressive increment of saline percentage and co 2 concentration. Screening and isolation of halophilic bacteria producing. Isolation and characterization of two novel halotolerant. Halophilic bacteria are important for numerous industrial processes. An aliquot 100 micro lit from decimal dilutions was inoculated on national botanical research institutes phosphate growth medium nbrip 11, and pikovskaya medium.
For references to other isolation methods see frankland et al. It was found that s8 isolate was an extreme halophile, s5 and s1 were. Isolation and application of salttolerant or halophilic psb will facilitate the development of salinealkali soilbased agriculture. Isolation of halophilic bacteria associated with saline and alkaline. Cultivable halophilic microorganisms were isolated and identified from saline and alkalinesodic soils.
Screening of halophiles from various saline habitats of india led to isolation of 108 halophilic bacteria producing industrially. Media for halophilic actinobacteria for ml distilled water. Isolation of phosphatesolubilizing bacteria from tomato and potato samples approximately 2 g of soil was scraped from the roots. Isolation of moderately halophilic bacteria from tunisian soil samples and transformation by electroporation using escherichia colihalomonas sp. Pdf cultivable halophilic microorganisms were isolated and identified from saline and alkalinesodic soils. Isolation and identification of halotolerant soil bacteria. Pdf isolation and characterization of halophilic bacteria. For the isolation of halophilic bacteria, 10 ml of sterile 14 strength ringers solution 2. Screening and isolation of halophilic bacteria producing industrially. Isolation and cultivation methods of actinobacteria. Soil sample collection for bacterial isolation, 10 g of soil was collected from different area within pudukottai district. If using the swabs, dip into the pbs and thoroughly agitate with the swab to release the bacteria.
The aim of this work was the isolation of cultivable halophilic bacteria from places with diverse salinealkaline characteristics. Isolation and characterization of bacteria isolated from municipal solid waste for production of industrial enzymes and waste degradation. In total, 52 actinomycetes were collected from 18 analyzed soil samples and were detected in both of the media used. A total five culture strains of bacteria and five fungal isolates were selected from all of the soil samples i. The aim of the study was to isolate and study the diversity among halophilic bacteria producing enzymes of industrial value. In this study of sunderban soil, focus has been made on the isolation of halophilic organisms and their characterisation. However, sayula and san marcos lakes have been poorly studied as source of microorganisms. To estimate the number of bacteria in a gram of soil, the soil must be both diluted and mixed thoroughly so that the aggregates are broken up such that a suspension of single cells is achieved. Isolation of halophilic and halotolerant bacteria from a. Isolation of moderately halophilic bacteria in saline. The novel strain was capable of growth at nacl concentrations of 115 % wv optimum at 510 % wv. Surface soils are a substrate of organic and inorganic particles forming a complex matrix surrounding pores that fill with air or water. Materials and methods collection of soil sample from saline zone area. Adaptability of the bacteria to such diverse conditions has im mense fundamental and applied importance.
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